Monofunctional Transglycosylases Are Not Essential for Staphylococcus aureus Cell Wall Synthesis
نویسندگان
چکیده
منابع مشابه
Monofunctional transglycosylases are not essential for Staphylococcus aureus cell wall synthesis.
The polymerization of peptidoglycan is the result of two types of enzymatic activities: transglycosylation, the formation of linear glycan chains, and transpeptidation, the formation of peptide cross-bridges between the glycan strands. Staphylococcus aureus has four penicillin binding proteins (PBP1 to PBP4) with transpeptidation activity, one of which, PBP2, is a bifunctional enzyme that is al...
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Major peptidoglycan transglycosylase activities, which synthesize uncross-linked peptidoglycan from lipid-linked precursors, were solubilized from the membranes of Staphylococcus aureus and Micrococcus luteus and were partially purified. The transglycosylase activities were separated from penicillin-binding proteins by solubilization and by purification steps. Therefore, we concluded that these...
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Inhibition of bacterial cell wall synthesis is an important mechanism of action of antibacterial agents. Bacterial cell walls are chemically and morphologically distinct from any structures found in animal cells. Inhibition of synthesis of these structures results, therefore, in selective toxicity. The antibacterial action of penicillins (1, 2), n-cycloserine (3, 4), bacitracin (5), and novobio...
متن کاملKinetic characterization of the monofunctional glycosyltransferase from Staphylococcus aureus.
The glycosyltransferase (GT) module of class A penicillin-binding proteins (PBPs) and monofunctional GTs (MGTs) belong to the GT51 family in the sequence-based classification of GTs. They both possess five conserved motifs and use lipid II precursor (undecaprenyl-pyrophosphate-N-acetylglucosaminyl-N-acetylmuramoyl- pentapeptide) to synthesize the glycan chain of the bacterial wall peptidoglycan...
متن کاملDirect observation of Staphylococcus aureus cell wall digestion by lysostaphin.
The advent of Staphylococcus aureus strains that are resistant to virtually all antibiotics has increased the need for new antistaphylococcal agents. An example of such a potential therapeutic is lysostaphin, an enzyme that specifically cleaves the S. aureus peptidoglycan, thereby lysing the bacteria. Here we tracked over time the structural and physical dynamics of single S. aureus cells expos...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2011
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.01474-10